Marker-Assisted Selection of Male-Sterile and Maintainer Line in Chili Improvement by Backcross Breeding

Cytoplasmic genic male sterility (CGMS) is a system that uses the maternal line for hybrid production, ensuring no obscurity of F1 seed purity and reducing cost production. Identification gene important plant improvement classification when using molecular marker-assisted selection (MAS) method. This study aimed to produce new (A-line) its maintainer (B-line) by transferring male-sterile from another variety restorer lines (C-line) achieve future In process, CGMS B-line transferred C1 C3 lines, which finally resulted in A-line (BC2F2A1 × C1, BC2F2A1 C3), (BC1F2B1 BC1F2B1 then used MAS method detecting genes pollen viability test newly improved lines. The results indicated 3336-last2-SCAR (1639 bp) 4162-SCAR (1046 DNA markers classified Rf locus, CMS-SCAR130/140 marker confirmed S or N cytoplasm. represented both male-fertile (SRf_) (Srfrf) progenies Mendelian ratio 3:1. Moreover, stained grains with 1% acetocarmine abnormal plants. detect (Nrfrf) are C1-14, C3-10, C3-11. successfully identified (Nrfrf), detected presence RfRf Rfrf genotype chilies at seedling stage. use these was highly accurate early generation stage conventional breeding program. It can be concluded were during backcross